Beauty-generated single-bacteria three-dimensional chemical image
Beauty-generated single-bacteria three-dimensional chemical image November 20, 2018 Source: Science and Technology Daily Author: Feng Weidong The US Department of Energy's Brookhaven National Laboratory uses ultra-bright X-rays to image higher-resolution individual bacteria, demonstrating an imaging technique called X-ray fluorescence microscopy (XRF) that can be used as a small creature. An effective method for sample 3D images. This achievement was published in the latest issue of the Science Report. Lisa Miller, a scientist at National Synchrotron Light Source II (NSLS-II), said that this is the first time that nanoscale XRF has been used to increase the resolution of bacterial imaging to cell membrane levels. Imaging cells at the membrane level is critical to understanding the role of cells in various diseases and developing advanced medical treatments. The record-breaking resolution of X-ray imaging benefits from the advanced features of the NSLS-II test bench, the hard X-ray nanoprobe (HXN) beamline, which features a novel nanofocus optical system and excellent stability. HXN is the first XRF beamline to generate a three-dimensional image using this resolution. Although other imaging techniques, such as electron microscopy, can also image the structure of cell membranes at very high resolutions, these techniques do not provide chemical information about the cells. Using HXN, a three-dimensional chemical map of the sample can be generated to determine the location of trace elements throughout the cell. The researchers used HXN to take sample images from different angles, each showing the chemical characteristics of the sample in that direction, and finally combining the profiles to create a three-dimensional image of the sample. The images produced by HXN show that the two trace elements calcium and zinc have a unique spatial distribution in bacterial cells. The researchers say that this three-dimensional chemical imaging or fluorescent nano-patterning technology has great application prospects in other scientific fields, such as helping to understand how the internal structure of the battery changes during charging and discharging. In addition to the technical barriers to breaking X-ray imaging resolution, the researchers have developed a new method for imaging bacteria in X-ray measurements at room temperature. The researchers said that demonstrating the efficacy of X-ray imaging and sample preparation methods is the first step in nanoscale imaging of trace elements in other biological cells. They are seeking to understand the subcellular location and function of metalloproteins in disease processes, such as the role of copper in neuronal death in Alzheimer's disease, to help develop effective treatments. Protective Clothings,Disposable Coveralls,Safety Protective Clothing,Disposable Protective Coveralls Xinxiang Huaxi Sanitary Materials Co., Ltd. , https://www.huaximedical.com