What should you pay attention to when installing and lubricating high-precision planetary reducers?
The planetary reducer is a device that uses a gear speed converter to decelerate the number of revolutions of the motor to the required number of revolutions and obtain a large torque. The installation and use of high-precision planetary reducers is directly related to its operation and must comply with technical specifications and standards. As a power transmission structure, the normal operation of the planetary reducer and the lubricating oil are inseparable, so in the process of use, special attention should be paid to the lubrication of the planetary reducer. The following Dongguan Ji Chuang Xiaobian will introduce the installation and lubrication precautions of high-precision planetary reducer for your reference. First, the installation 1. High-precision planetary reducers are generally required to be installed on a horizontal surface with an inclination of no more than 10°. If the inclination is greater than 10° due to special needs, please contact the company before use to discuss the matter. 2. When the input shaft and output shaft are connected with the power and supporting machinery, if the coupling is used, the shaft and the shaft are required to be strictly centered, and there must be no different heart phenomenon. The use of the V-belt and the chain should not be too tight or too loose. 3. The installation foundation should be firm and reliable, and there should be no vibration or looseness. The fixing screws should be selected strictly according to relevant standards and regulations. 4, the installation screws should be regularly checked whether it is tightened, fixed and secure, the gasket should use spring washers as much as possible. Second, lubrication 1. High-precision planetary reducer is generally lubricated with oil pool. Before use, be sure to add medium-pressure planetary oil N220 or N320 to the specified position (20-30mm on the low tooth) and replenish it regularly. 2, the use of lubricants should also pay attention to, do not add too full, easy to leak oil.
MLPA ( Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumour profiling.Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:
Denaturation > Hybridization Ligation > PCR MLPA Amplificons Capillary Electrophoresis >Data Analysis
PCR reaction consists of the three steps: denaturation, primer annealing and primer elongation. Those steps of PCR amplification are repeated many times. The fluorescence-labeled primers, which will go through the capillary electrophoresis to pass a detector, are incorporated into the size of the amplification products. The measured fluorescence was visualized as a peak pattern, the so-called electropherogram. The raw data from the capillary electrophoresis analyzer forms the input of the MLPA analysis.
The genetic analyzers of Superyears Gene can be used not only for Sanger sequencing but also for fragment analysis. Fragment analysis First, it obtains the fluorescence-labelled DNA fragments, performs the capillary electrophoresis using a Genetic Analyzer and compares the sample's relative size standard with the designed size standard markers through the analysis software.
The genetic analyzer of Superyears Gene can be used in the capillary electrophoresis during the process of the MLPA experiment, and the data obtained can be used for (compatible) professional analysis software.
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