Model NO.: Alpha-Amylase EnzymeHT-800
HS Code: 3507909000
Model NO.: Alpha-Amylase EnzymeHT-800
HS Code: 3507909000
Alpha-Amylase HT-800 is produced from Bacillus licheniformis using submerged fermentation and refining extraction technology. As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
HT-800 is a robust Heat-stable Alpha-Amylase designed for the production of starch sugar.
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Declared Enzyme: |
Alpha-Amylase |
Systematic Name: |
1,4-α-D-glucan glucanohydrolase |
Activity: |
≥80,000U/ml |
Appearance: |
Light to dark brown liquid |
Product pH: |
5.8 to 6.8 |
Specific gravity: |
1.15 to 1.25 g/ml |
Effect of pH
HT-800 is active with pH range 5.0 to 7.0, depending on the application. For maximum activity, the optimal pH for HT-800 is 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.
Effect of Temp
For interval liquefaction, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-800 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
Copper, titanium, and cobalt ions are moderate inhibitors for HT-800. Aluminum, lead and zinc ions act as strong inhibitors.
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For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, then add this product at a dosage rate of 0.10 to 0.35 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
In case the starch content is above 35%, recommended dosage rate is from 0.35 to 0.4kg/TDS.
Exact dosage rate depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
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This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided being stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen-months if this product is being store properly at 0~10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Material Safety Data Sheet.
Alpha-Amylase HT-800 is produced from Bacillus licheniformis using submerged fermentation and refining extraction technology. As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
HT-800 is a robust Heat-stable Alpha-Amylase designed for the production of starch sugar.
Â
Declared Enzyme: |
Alpha-Amylase |
Systematic Name: |
1,4-α-D-glucan glucanohydrolase |
Activity: |
≥80,000U/ml |
Appearance: |
Light to dark brown liquid |
Product pH: |
5.8 to 6.8 |
Specific gravity: |
1.15 to 1.25 g/ml |
Effect of pH
HT-800 is active with pH range 5.0 to 7.0, depending on the application. For maximum activity, the optimal pH for HT-800 is 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.
Effect of Temp
For interval liquefaction, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-800 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
Copper, titanium, and cobalt ions are moderate inhibitors for HT-800. Aluminum, lead and zinc ions act as strong inhibitors.
Â
For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, then add this product at a dosage rate of 0.10 to 0.35 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
In case the starch content is above 35%, recommended dosage rate is from 0.35 to 0.4kg/TDS.
Exact dosage rate depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
Â
This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided being stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen-months if this product is being store properly at 0~10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.
Inhalation of enzyme dust and mists should be avoided. In case of contact with skins or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Material Safety Data Sheet.
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