Wright-Gemsa dyeing solution

G1020 Wright-Gemsa dyeing liquid produced by Beijing Suo Laibao Technology Co., Ltd. is a composite dyeing solution, which combines the advantages of both Wright and Giemsa dyeing liquids, mainly used for blood and bone marrow smear dyeing. The various components of various cells and cells have different affinities for acid dyes and basic dyes in Wright-Gemsa staining liquid due to their different chemical properties. Therefore, specimen smears are stained by Wright-Gemsa After liquid staining, the corresponding types of cells exhibit different coloration, so as to achieve the purpose of distinguishing their morphological characteristics. Giemsa staining solution has strong cytoplasmic tincing ability, which can better show the basophilic degree of cytoplasm, clear coloration and pure color, but the coloration of the nucleus is deep and the nuclear structure is poor. Wright's staining has a moderate degree of coloration of the nucleus, clear nuclear structure and color, better recognition of the nuclear structure, but poor coloration of the cytoplasm, so the use of Wright's Giemsa mixed dyeing, with good dyeing effect, clear contrast, Easy to operate and so on.

Use of Wright-Gemsa dyeing solution:

This product can be used as a dye for a variety of tissues or cells. Different tissues, cells, and different uses can be used differently. For specific methods, please refer to the existing literature according to your own needs. This product takes smear as an example, for example, for reference only.

1. Take smear and dry naturally.

2. Add 2-3 drops of Wright-Gemsa dye solution to cover the entire specimen smear and dye for 1-2 minutes.

3. Add an equal amount of PH6.4 phosphate buffer (or equivalent amount of ultrapure water), gently shake the slide, mix well with Wright-Gemsa staining solution, and stain for 3-5 minutes.

4, washing, blotting, microscopic examination.

5. After staining, the staining of cytoplasm and nucleus is clear and distinct. The nucleus is colored in different shades of purple, the cytoplasm is light red, and the granules are clearly distinguished.

Precautions:

1. The thickness of the smear is suitable, and the smear is fixed after drying, otherwise the cells will fall off easily during the dyeing process.

2. The dyeing solution should not be too small to prevent evaporation and precipitation of the dye. The rinsing time should not be too long to prevent discoloration.

3, dyeing is very sensitive to pH, the dilution of the dye solution must use buffer, rinse water should be close to neutral, otherwise it may lead to abnormal cell staining, the shape is difficult to identify, or even wrong.

4. If the dyeing is too light, it can be counterstained. When counterstaining, buffer should be added first, then the dyeing solution should be added. If the dyeing is too deep, it can be rinsed or soaked with running water, or it can be decolorized with methanol.

Wright-Gemsa dyeing products:

P2100 10× polylysine

G1010 Giemsa staining solution

G1040 Wright's dye solution

G1100 Eosin Staining Solution

G1140 Hematoxylin staining solution (conventional staining)