Veterinary drug residue detection method

Veterinary drug residue detection method: veterinary drug residue detection includes antibiotics, sulfonamides, hormones, and veterinary drug residues such as furazolidone and nifurate. The veterinary drug residue detection method currently has the following detection methods:

1. Gold standard detection method (gold standard detection card)

Colloidal gold is a commonly used labeling technique. It is a novel immunolabeling technique applied to antigen antibodies by colloidal gold as a tracer marker, and has its unique advantages. It has been widely used in various biological research in recent years. Immunoblotting techniques used in clinical practice almost all use their markers. It can be used in flow, electron microscopy, immunity, molecular biology and even biochips.

2, enzyme-linked immunosorbent assay

The enzyme-linked immunosorbent assay, referred to as enzyme-linked immunosorbent assay, or ELISA; its center is to allow antibodies to bind to the enzyme complex and then detect by color development. The rapid detection instrument for veterinary drug residue CSY-E96SY veterinary drug residue rapid detector adopts the principle of solid-phase enzyme-linked immunosorbent ELISA, ie, enzyme-linked immunosorbent assay; quantitative detection of amoxicillin, malachite green, sulfonamides, Rheumatoid, disease diagnosis, melamine test, enrofloxacin, ciprofloxacin, erythromycin, chloramphenicol, oxytetracycline, tetracycline, sulfonamides (total), olaquindox, diethylstilbestrol, etc. Detection of toxic and hazardous substances and antibiotic residues. And can be connected to the food safety monitoring system. The veterinary drug residue rapid detector is widely used in farms, slaughterhouses, deep processing enterprises of meat products, and inspection and quarantine units.

3. High performance liquid chromatography

Also known as "high pressure liquid chromatography", "high-speed liquid chromatography", "high resolution liquid chromatography", "modern column chromatography" and so on. High performance liquid chromatography (HPLC) is an important branch of chromatography. With a liquid as the mobile phase, a high-pressure infusion system is used to pump a mobile phase with a single solvent of different polarity or a mixture of different solvents and buffers into a stationary phase. The column is separated from the components in the column and then entered into the detector for detection, thereby realizing the analysis of the sample. This method has become an important separation and analysis technology application in the fields of chemistry, medicine, industry, agronomy, commodity inspection and legal inspection.

4, Gas Chromatography

Gas chromatography can be divided into gas-solid chromatography and gas-liquid chromatography. Gas-solid chromatography refers to a chromatographic separation method in which the mobile phase is a gas and the stationary phase is a solid material. For example, activated carbon, silica gel or the like is used as a stationary phase. Gas-liquid chromatography refers to a chromatographic separation method in which the mobile phase is a gas and the stationary phase is a liquid. This is a new separation and analysis technology that has been widely used in industry, agriculture, national defense, construction, and scientific research. Gas chromatography can be divided into gas-solid chromatography and gas-liquid chromatography.

5. Mass Spectrometry (MS)

The use of electric and magnetic fields to move ions (charged atoms, molecules or molecular fragments, molecular ions, isotope ions, fragment ions, rearranged ions, multi-charged ions, metastable ions, negative ions and ion-molecule interactions) The generated ions are separated by their mass-to-charge ratio and then detected. The exact mass of the ion is measured to determine the composition of the compound of the ion. This is because the exact mass of the nuclide is a multiple number of decimals. The quality of the two nuclides will never be the same, and there will never be one nuclide whose mass is exactly an integer multiple of the mass of the other nuclide. Analysis of these ions yields information on the molecular weight, chemical structure, cleavage pattern of the compound, and some interrelationship between certain ions formed by the decomposition of a single molecule.