Refrigerated centrifuge use and instrument equipment precautions

There are many varieties of centrifuges, which can be divided into low-speed centrifuges, high-speed centrifuges and ultra-high-speed centrifuges according to the number of revolutions. Low-speed centrifuges with a number of revolutions less than 10000r/min are high-speed with a number of revolutions of 10,000-30000r/min. Centrifuge, the number of revolutions greater than 30,000 r / min is ultra-high-speed centrifuge; according to the temperature control can be divided into refrigerated centrifuge and ordinary temperature centrifuge, refrigerated centrifuge with refrigeration system, can control the temperature to the lowest - 20aC, ordinary centrifuge without refrigeration system;

<br> centrifuge equipment and use centrifuges divided into filter centrifuge and decanters two categories.

The main principle of the filter centrifuge is to accelerate the liquid phase in the solid-liquid mixture to the drum by the centrifugal force generated by the high-speed centrifugal drum (with appropriate filter material), and leave the solid phase in the drum. , to achieve the effect of separating solids and liquids, or commonly known as dehydration.

The main principle of the decanter centrifuge is to accelerate the sedimentation velocity of different specific gravity components (solid phase or liquid phase) in the mixture by the powerful centrifugal force generated by the high-speed rotation of the rotor, and to separate the different sedimentation coefficients and buoyant density substances in the sample. The centrifuge commonly used in the laboratory is a decanter centrifuge.

3H24RI intelligent high speed refrigerated centrifuge

The electric centrifuges commonly used in physical and chemical experiments include low-speed, high-speed centrifuges and low-speed, high-speed refrigerated centrifuges. Among them, low speed (including large capacity) centrifuges and high speed refrigerated centrifuges are most commonly used.

1. Ordinary (non-frozen) centrifuges are simple in structure, and can be divided into small benches and floor-standing models, equipped with drive motors, governors, timers and other devices. The speed of low-speed centrifuges generally does not exceed 10,000r/min, and the maximum number of revolutions of Hershey floor-standing high-speed centrifuges can reach 26000r/min.
2, high-speed refrigerated centrifuge such centrifuge speed can reach more than 20000r / min, in addition to the performance and structure of low-speed refrigerated centrifuge, the angle rotor used in high-speed centrifuge is made of titanium alloy and aluminum alloy. The centrifuge tube is a polyethylene hard plastic product with a cover. Such centrifuges are often used to collect microorganisms, cell debris, cells, large organelles, sulfate precipitates, and immunoprecipitates.

The low-speed refrigerated centrifuge is an ideal device for separating platelet separation and radioimmunoassay of particles of different densities. Low-speed refrigerated centrifuges are mainly used in medicine, chemistry, biology, biological products, agricultural science, food research and biochemical products, scientific research, food safety, medical and health, blood stations, hospital medicine technology education and production departments, molecular biochemistry, sanitation and other industries. And the production unit for precipitation separation of various suspension samples.

Low-speed refrigerated centrifuges are the most commonly used in laboratories for the extraction of biological macromolecules, sediments, etc. The rotating head is mostly made of aluminum alloy, flat and angled. The centrifugal tube has various types of hard glass, polyethylene hard plastic and stainless steel tube. The centrifuge is equipped with a drive motor, timer, regulator (speed indication) and refrigeration system (temperature adjustable range -20~+40°C), which can be changed according to the needs of centrifugal materials. .

Hershey's low-speed refrigerated centrifuges are made of high-quality materials and are subjected to centrifugal force for all transmission components, all with a high safety factor. All of its rotating parts are housed in the machine and equipped with a shock absorber for smooth operation. There is steel plate protection outside the centrifuge chamber and an organic cover to ensure safe use. The metal parts of the centrifuge are treated for oxidation or paint surface preservation. The centrifugal chamber is made of stainless steel, which has good corrosion resistance and durability. The operation control of the centrifuge is concentrated on the operation table on the right side of the fuselage table, which is easy to operate and simple to use.

TDL6M desktop low speed refrigerated centrifuge

Use of low-speed refrigerated centrifuges and precautions <br> Centrifuges are often used in physical and chemical experiments. To ensure smooth and safe operation of the experiment and to extend the life of the equipment, the experimenter must follow the following procedure.
(1) Inspection of the equipment before the start of the experiment, including checking whether the bolt is loose, whether the drum is firmly connected to the spindle, whether the brake device is complete and effective, and checking whether the power supply is reliable or not.
(2) Take two centrifuge tubes, weigh them on the balance, and adjust the amount of material in the tubes so that the weights of the two tubes together with their sleeves are equal. If there is only one material, the water can be balanced in the opposite one. Place the centrifuge tube containing the material in the metal sleeve of the centrifuge, and if necessary, pad a layer of cotton on the bottom of the tube.
(3) Place the centrifuge tube and its casing into the centrifuge moving plate in a symmetrical position, and cover the cover.
(4) Turn on the power and slowly rise to the set speed for a certain period of time.
(5) After the end of the centrifugation, the speed slowly drops to reach the speed zero. At this time, the cover can be opened by pressing the stop button.
(6) After the mobile disk automatically stops rotating, the centrifuge cover can be removed and the centrifuge tube removed. When taking out the centrifuge tube, care should be taken not to cause the precipitated material to rise due to vibration and turbidity.
(7) When using a centrifuge, if the centrifuge vibration is developed and there is noise, the internal weight is unbalanced. If a metal tone is found, it often indicates that the internal test tube is broken, and should be stopped immediately for inspection.
(8) Final inspection, cut off the power supply.