Rat (NAP-2/CXCL7) ELISA kit technology principle
Disposable gloves are available in latex and synthetic materials, in sterile and non-sterile forms and with and without powder. Sterile disposable gloves should be worn during invasive procedures and when the hands are in contact with wounds. Non-sterile disposable gloves are recommended when the hands are in contact with blood and other body fluids and for indirect contact with wounds.
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Product ID: QY-SZ2079
Product traits: liquid product use: scientific research experiment product specifications: 96T/48T
Customer Service Phone: 021-51867124 QQ Email @qq.com
Experimental principle <br>The microplate is coated with purified antibody to prepare a solid phase carrier, and the sample or standard, biotinylated anti-antibodies, HRP marker are sequentially added to the micropores coated with the antibody against the index. The avidin, after thorough washing, was developed with the substrate TMB. TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with this indicator in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration.
Kit composition:
Sealing film: 2 pieces (48) / 2 pieces (96)
Instructions: 1 sealed bag: 1 standard: 2700ng/L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8°C Preservation enzyme labeling plate: 1×481×962-8°C Preservation sample dilution: 3ml ×1 bottle 6ml×1 bottle 2-8°C Preservation developer A solution: 3ml×1 bottle 6ml×1 bottle 2-8°C Preservation developer B solution: 3ml×1 bottle 6ml×1 bottle 2-8°C Stop solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C Preservation concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation
Collection and preservation of specimens
1. Serum: Whole blood samples should be placed at room temperature for 2 hours or 4 °C overnight, centrifuged at 1000 xg for 20 minutes, the supernatant can be detected, or the specimens should be stored at -20 ° C or -80 ° C, but should be avoided. Freezing and thawing.
2. Plasma: EDTA or heparin can be used as anticoagulant. Centrifuge at 2 - 8 ° C 1000 xg for 15 minutes within 30 minutes after collection, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing. melt.
3. Cell culture supernatant or other biological specimens: centrifuge at 1000 xg for 20 minutes, take the supernatant for testing, or store the specimen at -20 °C or -80 °C, but avoid repeated freezing and thawing.
Note: Specimen hemolysis will affect the final test results, so hemolysis specimens should not be tested.
4. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When the components in the cells were detected, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
5. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. A certain amount of PBS (pH 7.4) was added, and the specimen was homogenized by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use.
6. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.
7. Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
Rat (NAP-2/CXCL7) ELISA kit technology principle Our products are provided with instructions, free of charge.
If it is not possible to judge by experimental data only, you can send the kit back to us for post-sale testing.
If it is the quality of the kit itself, you can return it for you;
If it is a problem with the experimental operation, the technician can give you some suggestions for improvement. Tips: Not for clinical treatment. 
