Application of 101bio exosome extraction kit in exosomes with very low albumin content
Exosomes are an extracellular vesicle that most normal and diseased cells can secrete, with a particle diameter of between 30 and 150 nm. Exosomes are widely found in body fluids such as plasma, lotion, urine, saliva, etc., and can carry a variety of proteins, lipids, DNA, RNA and other substances in the mother cells, involved in cell communication, cell migration, angiogenesis and tumor cells. Growth and other processes. Greenhouse Dutch Buckets,Dutch Bucket,Tomato Dutch Bucket,Plastic Dutch Bucket JIANGSU SKYPLAN GREENHOUSE TECHNOLOGY CO.,LTD , https://www.greenhousehydroponic.com
Exosomes were first recorded in the 1880s, when people only used it as a kind of cellular waste. The 2013 Nobel Prize in Physiology or Medicine was awarded to scientists such as James E. Rothman for their discovery of the regulatory mechanisms of vesicle transport. In recent years, due to the special nature of exosomes, researchers have been enthusiastic about the study of exosomes, and found that it has great potential in the field of diagnosis and treatment.
In the study of exosomes, the separation and extraction of exosomes has been a major problem that has plagued researchers. The ultracentrifugation extraction method established by Clotilde Théry, a senior scientist in the field of exosomes, has been regarded as the "gold standard" for exosomes extraction. However, the ultracentrifugation method requires a large sample size, takes a long time, relies on a specific instrument, has a low extraction amount, and has low defects such as low purity of the exosomes, which greatly reduces the research efficiency of the researcher. With the continuous development of exosome research, some commercial kits have emerged. Most of these kits use the principle of PEG precipitation, and the extraction can be completed in about half an hour. However, due to the addition of PEG during the extraction process, the obtained exosomes contained PEG conjugates, which seriously affected the subsequent experiments. In addition, whether it is ultracentrifugation or PEG precipitation kit, the isolated exosomes contain a large amount of albumin, which also causes great interference to subsequent experiments.
In response to the problems encountered in the exosome extraction process, 101bio USA developed and produced an exosome separation kit based on density gradient separation. The kit eliminates the need for ultracentrifugation relative to density gradient centrifugation, and also utilizes the specific polarity and charge characteristics of the exosomes, which is much simpler than conventional density gradient centrifugation and yields much higher. The most important thing is that the exosome albumin extracted by comparing the 101bio exosome extraction kit is much lower than the exosomes extracted by the ultracentrifugation and PEG precipitation kits. Cleared a big obstacle. In addition, by particle size analysis, the diameter of the particles extracted by the 101bio exosomes extraction kit was as high as 96.3% between 30 and 150 nm, indicating that the exosomes extracted by the kit contained almost no other types of particles.
Based on different samples used by different researchers, 101bio has developed kits for cell culture supernatants, plasma/serum, stem cell culture supernatants, urine and other body fluids. For specific products and other related information, please call 101bio China exclusive agent Beijing Ximeijie Technology Co., Ltd. 010-88497838 or visit the official website of Ximeijie http:// for consultation.